Aminopeptidase N (AP-N)
Links to databases
AP-N is a broad specificity aminopeptidase. It may play a critical role in the pathogenesis of cholesterol gallstone disease, and may be involved in the metabolism of regulatory peptides of diverse cell types, such as angiotensin III and IV, neuropeptides, and chemokines. It is found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Furthermore, it is s implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. AP-N is used as a biomarker for acute myeloid leukemia and plays a role in tumor invasion, as well as used as a biomarker?to detect damage to the?kidneys, and may also be used to help diagnose certain?kidney disorders. AP-N also mediates cytomegalovirus (HCMV) infection.
Sample type
Recommended sample types are EDTA plasma and serum. A range of additional sample types are compatible with the technology (PEA), including citrate plasma, heparin plasma, cerebrospinal fluid, (CSF), tissue and cell lysates, fine needle biopsis, microdialysis fluid, cell culture media, dried blood spots, synovial fluid, saliva, plaque extract and microvesicles. Please note that protein expression levels are expected to vary in different sample types. Certain assays are differentially affected by interfering substances such as hemolysate. Download any of our Data Validation documents or contact support@olink.com for more information.
Analytical Measuring Range
Please note: the technical data reported below refers to the measured value in the in vitro validation assays. The calibrator curve below shows the performance of the assay with the estimated sensitivity and dynamic range parameters indicated. These curves are generated during the assay validation process using recombinant antigens. Please note that when analyzing biological samples the data generated will be given in the form of relative quantification (NPX values) and cannot be converted to absolute protein concentrations. For more info about NPX measurements, please visit our FAQ page (https://www.olink.com/question/what-is-npx).
Since the CVD III panel uses human samples diluted 100-fold, a multiplication factor of 100 should be applied when comparing the addressable biological concentration to this validation data.
- LOD (pg/mL)
- 61.04
- LLOQ (pg/mL)
- 122.07
- ULOQ (pg/mL)
- 125000
- Hook (pg/mL)
- 125000
- Range (logs)
- 3
Calibrator curve for validation data (generated in multiplex)
Precision
- Within run precision Coefficient of Variation (CV)
- 7%
- Between run precision Coefficient of Variation (CV)
- 8%
Precision (repeatability) is calculated from linearized NPX values over LOD.
Biomarker Validation Data
Additional validation data, as well as a more detailed description of how the Olink panels are quality controlled can be found in our Data Validation documents. To download or to learn more go to the Data Validation page.
